Amaroli Andrea

Steering Multipotent Mesenchymal Cells towards an Anti-inflammatory and Osteogenic Bias through Photobiomodulation Therapy: Achieving Dual Benefits

The multipurpose features and clinical applications of bone marrow-derived multipotent mesenchymal cells (MSCs) have captured scientific interest. MSCs not only exert bone-building and niche anabolic tasks but also possess the ability to quench inflammation and restore inflamed tissues, extending their range of activities to conditions such as neurodegenerative diseases, immune disorders, and various forms of osteopenia. Manipulating MSCs to become an effective therapeutic tool has become necessary. Photobiomodulation therapy (PBM-t) can be used to render MSCs an extraordinary anti-inflammatory and osteogenic instrument by improving their plasticity and secretome. Laser pre-conditioning and PBM-t protocols improve the anti-inflammatory and osteogenic capacity of MSCs, prompt these multipotent stem cells towards a predefined lineage commitment and secretome, and improve their viability and proliferation. Consistent therapeutic approaches are essential in considering the physical properties of light, the reliability of laser parameters, and individual patient needs. The field needs to shift from pre-clinical evidence to reliable therapies, which involve determining the effectiveness, safety, posology, and clinical utility of PBM-t protocols. Our research team suggests that delivering 808-nm infrared light at 1W and 60 J/cm2 (60 sec) through a flat-top handpiece (1 cm2) is a promising strategy for improving MSC differentiation, secretion, and anti-inflammatory capabilities, establishing the clinical utility of this approach.