Adriana Barylyak

Associate Professor, Ph.D.

Department of Therapeutic Dentistry, Danylo Halytskyi Lviv National Medical University

Head of the Department of Laser Dentistry Center of implantation and prosthetic dentistry MM

Visiting professor of Sapienza University, Rome, Italy

In 2004  graduated with honors from the Faculty of Dentistry of Lviv National Medical University. In 2005 master’s degree in medicine. Since 2006 worked at the dental clinic of the Medical University of Vienna under the Ernst Mach Scholarship Program and the agreement between LNMU. Danylo Halytsky, Technical University of Vienna and Medical University of Vienna (Austria). In 2009 she defended her PhD thesis on “Nanolaser disinfection of the root canal system.” Was a superwiser of the project “Substantiation and experimental development of nanolaser therapy of dentin hypersensitivity”, GP / F44 /, 2012 (grant of the President of Ukraine to support research of young scientists). Since 2007 member of the World Association of Laser Technology in Dentistry (SOLA), a graduate of the SOLA Academy of Laser Dentistry (Vienna). Since 2009 – Head of the Department of Laser Dentistry of the Center for Dental Implantation and Prosthetics “MM”.

Practical dental experience: general periodontology, conservative dentistry, laser dentistry.

Author of over 90 scientific papers. Member of the editorial board of the journal “Osteology, Implantology, Periodontology”, “International laser Magazine”.

President of the Society of Laser Dentistry of Ukraine, Member of the Board of the World Federation of Laser Dentistry (WFLD-ED), SILO, Ukrainian Academy of Periodontology, a full member of the National Union of Dentists of Ukraine.

Main areas of activity: periodontology, aesthetic dentistry, nanotechnologies, the use of different types of lasers in dentistry, photodynamic therapy in the treatment of periodontal tissues, peri-implant tissues, oral mucosa.

Сo-organizer of a number of specialized conferences in Ukraine, invited speacker and moderator at international congresses (Bruges, Belgium 2008, Vienna, Austria 2010, Rome, Italy 2010, London, UK 2013, Krakow, Poland 2015, 2017, 2019, Thessaloniki, Greece 2017, Aachen, Germany 2018, Poznan, Poland 2018, Parma, Italy 2019, Montpellier, France 2021. Also is a speaker and trainer of dental workshops in Ukraine (Lviv, Kyiv, Odesa, Dnipro, Kharkiv, Mykolaiv)

Title: Laser nanotechnologies in treatment of dentin hypersensitivity

Dentin hypersensitivity (DH) is one of the most common complications that affect patients after periodontal therapy. So far, many investigators have successfully used different types of laser on DH treatment.

The proposed method envisages treatment of dentine hypersensitivity by application of nanoparticles onto tooth sensitive surface. The method is distinguished by the use of nanoparticles of calcium fluorine apatite (CaFAP) and irradiation by CO2 and YAG:Er lasers.

Development of effective treatments for dentin hypersensitivity by using nanolaser technologies.

Sixty extracted human teeth with dentine hypersensitivity were stored in the physiological saline solution, were assigned to the three different experimental groups. The first group was control. The samples in the second group were covered with nanodispersed fluorine apatite and irradiated with Er:YAG laser (0.5 W, 50Hz, 5 times x 5s). The samples in the third group were covered with Nanodispersed fluorineapatite and irradiated with CO2 laser (0.5 W, 5s, 5 times). The samples were cut longitudinally with a diamond-coated band saw (“Trennschleif System”, Exakt, Norderstedt, Germany) then studied under the scanning electronic microscope.

A group of nine patients with a total of 63 chronic hypersensitive teeth were selected. Each one of them should at least have three hypersensitive teeth. These teeth were randomly allocated into three groups. Group 1, control; group 2, teeth were covered by nanodispersed fluorine apatite and irradiated with Er:YAG laser (0.5 W, 50Hz, 5 times x 5s); and group 3 teeth were covered with Nanodispersed fluorineapatite and irradiated with CO2 laser (0.5 W, 5s, 5 times)

Assessment of pain was performed by a visual analysing scale (VAS) after stimulation of sensitive tooth by using the sharp tip of an explorer. This test was performed before treatment, immediately after that and at 1-, 3- and 6-month intervals after treatment by one blinded examiner. Analysis of VAS score between the three groups at the time of treatment did not show any significant difference (p = 0.506). However, by using repeated-measurement analysis of variance test, significant differences were seen in the three groups between before-treatment VAS score and after treatment (p < 0.0005).

Fluorine apatite nanoparticles penetrate into open dentinal microtubules and make obturation. The next laser irradiation induce photothermal activation, the melting of nanoparticles. The wavelength of laser irradiation lies at the maxima of the spectral absorption of calcium fluorineapatite (CO2 laser – 10.6 microns or Er: YAG laser is 2.94 microns). The energy regimes of laser irradiation were determined by computer simulation correlated with experimental and clinical results.

The proposed method provides reliable, durable and natural CaFAP adhesion in microtubules orifices – obturation and effective re-mineralizing function on the hypersensitive dentin surface.